Becker & Hickl
Becker & Hickl DCS-120 MP Multiphoton FLIM Confocal System
This unique bespoke system brings together a wide range of cutting-edge techniques that are not commonly available, all within a single platform based on a Zeiss Axio Observer 7 inverted microscope. Its core innovation is the combination of tunable pulsed excitation from a femtosecond laser source (Insight X3, Spectra-Physics), an acousto-optic modulator and a second harmonic generation module (APE HarmoniXX Select SHG) with single photon counting detectors and time-correlated single photon counting (TCSPC) electronics from Becker & Hickl.
The counting electronics have an exceptionally fast time response (<40 ps, compared to 100 ps on the previous FLIM system), allowing quantitative determination of fluorescence observables with unprecedented accuracy. This makes the system a powerful and versatile platform for advanced lifetime, anisotropy and correlation-based imaging.
-
Excitation: femtosecond pulsed laser (Insight X3) with AOM and SHG module (HarmoniXX Select)
-
Detectors: 2 x single photon counting detectors (HPM-100-07) on the confocal and non-descanned channels
-
TCSPC electronics: SPC-180NX, time response <40 ps
-
Objectives: 4x, 20x, 40x and 63x oil
-
Fluorescence lifetime imaging (FLIM) with both 1P and 2P excitation (340–650 nm and 680–1300 nm)
-
Time-resolved fluorescence anisotropy imaging (trFAIM)
-
Phosphorescence lifetime imaging (PLIM)
-
Fluorescence correlation spectroscopy (FCS)
-
Second harmonic imaging (SHIM)
-
Non-descanned detection for deep-tissue multiphoton imaging
