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Renishaw inVia Qontor upright confocal raman microscope

The Raman_InVia microscope allows users to interrogate chemical or molecular differences in cells and tissues. Raman microscopes rely on the differences in the way molecules--nucleic acids, protein, lipids, metabolites, polysaccharides etc.--scatter light. Raman spectroscopy can detect these differences or “fingerprints”, allowing users to see intracellular distributions of molecules without labelling or staining. Examples of successful applications of Raman imaging include identification of stem cells from a mixed cluster of cells, measuring the efficiency of drug uptake with cells, or following metabolite distributions within organelles over time in 3d.

•532nm, 632nm, 785nm, and 833nm laser lines •Two cameras: Renishaw internal CCD and Andor Newton emCCD •Objectives:5x, 20x, 50x LWD, 60x W and 100x. •high spatial resolution--just under a micrometre in xy •Automated xy stage for scanning samples. •LiveTrack automatically keeps focus over rough surfaces •Streamline feature allows fast scanning of sample with a line shaped laser •Pinhole allows confocal sectioning •WiRE software for data processing and analysis

Example of Raman
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Example Raman
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