Elyra7SIM
Zeiss Elyra 7 inverted widefield microscope with Lattice SIM, apotome, TIRF and SMLM
The Elyra7SIM is a dual camera super-resolution microscope with a variety of imaging modes: fluorescent widefield, Apotome structured illumination (SIM), Lattice SIM, TIRF, and single molecule localisation microscopy (SMLM)--STORM, PALM. In SIM modes, the sample is illuminated with patterned light--stripes or a lattice of spots--multiple images are acquired, which allow out of focus light removal. Lattice SIM generates super-resolution images (120nm xy, 300nm in z). Both SIM modes are excellent for live imaging, allowing fast imaging of large fields of view, with low light levels. If speed is your limiting factor, the Elyra 7 can deliver optically sectioned images much faster than scanning confocals. If you need even more resolution, the Elyra 7 allows super-resolution imaging down to 20nm in xy and 50nm in z, using techniques like PALM and STORM. It has proven particularly useful for small model organisms, c. elegans, yeast, bacteria, algae as well as cells in culture.
•Laser lines: 405, 488, 561, 642nm. •dual sCMOS cameras for simultaneous acquisition of two channels •Objectives: 10x, 20x, 40x W, 40x oil, 63x oil. •Collect z-stacks 3x faster using Leap mode. •Burst mode allows acquisition at up to 255 frames per second. •Incubator and CO2 for live cell imaging.